STMS (sequence tagged microsatellite site) molecular markers as a valuable tool to confirm controlled crosses in chickpea (Cicer arietinum L.) breeding programs

Abstract

Chickpea (Cicer arietinum L.) is an annual, diploid and autogamous grain legume. Most breeding processes developed in this crop start with complementary crosses, but artificial hybridization is a tedious operation probably due to the injury of the pistil caused during emasculation due to the small size of the flowers. The success in pod formation after hybridization together with the possibility of testing the hybrid nature of F1 is a key point to optimize chickpea breeding. Morphological characters are not always adequate to distinguish between progeny, consequently molecular markers are needed to verify hybrid nature. In this study, we show our experience in crossing programs and the use of molecular markers to test hybridization rate in three different cases (A) obtaining advanced lines combining resistance to blight and fusarium wilt; (B) developing segregating populations for Orobanche foetida and (C) providing suitable materials to do genetic studies involving double pod mutants. A total of 2041 pollinated flowers produced 21.7, 16.8 and 8.7% of pods in each one of the three cases analyzed. Hybridization nature in F1 seeds was tested with STMS (sequence tagged microsatellite site) markers and ranged from 86 to 91% for the three cases. STMS markers were a valuable tool to detect hybrids, being simple to visualize and having a low cost. The confirmation of hybrid nature at the initial development stages of F1 plants is critical for reducing time and costs in breeding programs.