Preliminary study of the effect long storage of whole blood cell using anti-coagulant EDTA on blood impedance by the electrical impedance spectroscopy metho

Abstract

Hematology is a series of blood tests carried out to identify any problems or blood disorders based on the number of cells.There is a tendency to postpone hematological examinations because of the high demand for samples to be tested. Delayed analysis of blood samples can affect the reliability and validity of hematological testing results. This research aims to determine the characteristics of the blood impedance value stored in an EDTA tube for 6 hours based on the electrical impedance spectroscopy method. The number of blood cells factor influences the characteristic of blood impedance. Measurement of whole blood cell impedance was injecting 100 μA currents into the sample at the frequency range of 1 Hz to 1 MHz. The longer the blood is stored, the higher the blood impedance value. The condition happens because of changes in blood morphology during storage. The effect of blood storage time shows a shift in the characteristic impedance value after 2 hours of storage.