PD-1 deficiency results in the development of fatal myocarditis in MRL mice

Abstract

The deficiency of programmed cell death 1 (PD-1, Pdcd1), a negative immuno-receptor belonging to the CD28/cytotoxic T lymphocyte antigen 4 (CTLA-4) family, can support various tissue-specific autoimmune conditions. Here, we analyzed the effect of PD-1 deficiency in MRL mice that is genetically predisposed to systemic autoimmunity. MRL-Pdcd1 -/- mice developed a fatal myocarditis, which is reminiscent of CTLA-4-deficient (Ctla4 -/-) mice. Massive infiltration of CD4 + and CD8 + T cells and myeloid cells was found in hearts of MRL-Pdcd1 -/- mice concomitant with the production of high-titer auto-antibodies against cardiac myosin. In contrast to Ctla4 -/- mice in which most of the CD4 + T cells are non-specifically activated and invade various organs, T cells in the heart but not in the spleen and lymph nodes are activated in MRL-Pdcd1 -/- mice, suggesting that myocarditis is mediated by antigen-specific autoimmune response. Heart infiltrating myeloid cells strongly suppressed the allogenic response of T cells in vitro, suggesting that these Mac1 + Gr1 + myeloid cells are phenotypically similar to myeloid suppressor cells, which can be found in tumorbearing hosts. These findings unravel the hidden heart-specific autoimmune predisposition of MRL mice and provide MRL-Pdcd1 -/- mice as a useful animal model of lymphocytic myocarditis. © The Japanese Society for Immunology. 2010.