Investigations on transforming Triticum aestivum via the pollen tube pathway

Abstract

Attempts were made to optimize the necessary conditions to transform wheat Triticum aestivum using the pollen tube pathway. Three methods previously described as successful in cereals and plasmid constructions carrying either NPT II or GUS markers were tested. Functionality of GUS expression vector was checked by particle gun experiments on wheat leaves. Experiments described in this paper show that a Tris-HCl-150 mM pH 9 solution containing 0.2 M MgSO4 inhibits both stigma and pollen nucleases. However, none of the tested plants among the 2 731 screened for GUS activity or for their resistance to kanamycin, expressed the reporter genes. © 1992.