Prognostic significance of matrix metalloproteinase-2, -8, -9, and -13 in oral tongue cancer

Abstract

Background: Oral tongue squamous cell carcinoma (OTSCC) often metastasizes to cervical lymph nodes. Mechanisms of this disease progression are not fully known. We aimed at finding new predictive markers for diagnosis and disease monitoring. Methods: Seventy-three consecutive T1N0M0 and T2N0M0 OTSCC patients treated at Helsinki University Central Hospital, Helsinki, Finland, in 1992-2002 were included. Tissue array blocks were prepared from primary tumors and immunostained. Immunoexpression of matrix metalloproteinase (MMP)-2, -8, -9, and -13 was compared with patient characteristics and outcome. Results: Nuclear expression of MMP-13, but not cytoplasmic expression of MMP-2, -8, and -9, was associated with invasion depth (P=0.017) and tumor size (P=0.008). Furthermore, high nuclear MMP-13 expression was predictive of poor outcome (P=0.042). Conclusion: Our results suggest that especially MMP-13 may be regarded as a prognostic biomarker in OTSCC. © 2011 John Wiley & Sons A/S.