Characterization of Temperate Bacillus Bacteriophage φ105

Abstract

Temperate Bacillus phage φ105 is serologically unrelated to previously described virulent Bacillus phages. Phage φ105 is incapable of generalized transduction. Prophage φ105 is inducible with mitomycin C. Phage φ105 contains double-stranded deoxyribonucleic acid (DNA) with a molecular weight of about 25 × 10 7 as determined by band sedimentation and electron microscopy. The per cent guanine plus cytosine of φ105 DNA is 43.5 as determined by buoyant density in CsCl and by thermal denaturation. Phage φ105 DNA may contain complementary single-stranded ends.