Comparison between PCOS and Non-PCOS women regarding follicular fluid IL-10,IL-12, IL-17A and IL-23 levels

Abstract

This study was conducted on the patients attended special private clinics for the treatment of infertility in Baghdad city for the period from the end of July 2015 until the end of September 2016 for estimation of interleukin (IL)-10, IL-12, IL-17A and IL-23 in in the follicular fluid of "polycystic ovary syndrome (PCOS) women.The study included 55 women with polycystic ovary syndrome and 35healthy women. The follicular fluid was collected from the patients and the control group during the ovulation period, i.e. 12-14 days of the menstrual cycle, 3 ml of follicular fluid were collected by physicianand then kept in the refrigerator freezing until for estimation oflevel of interleukin -10, IL-12, IL-17A and IL-23 in in the follicular fluid of "PCOS women" by using ELISA technique (Quantikine Co. USA).The current study showed that most PCOS, who reached the age of twenty to twenty-nine years and 60%of them andexcess weight. All polycystic ovarian women were all affected by the appearance of hair in the bodies here (hirsutism). Study also showed that 65.45% ofPCOScases were suffering from the appearance of acne in their skin and majority of cases were with irregular menstrual cycle and positive family history of the syndrome and 74.55% were with alopecia"."The study demonstrated thatIL-10 was significantly elevated in PCOS women" (P. value 0.0004) "as compared with healthy women" (33.8±3.71 vs. 17.5±2.84pg/ml)"."The study demonstrated thatIL-12 was significantly reduced in PCOS women" (P. value 0.0012) "as compared with healthy women" (1.26±0.02 vs. 3.09±0.011 pg/ml)"."The study demonstrated thatIL-17A was significantly elevated in PCOS women" (P. value 0.001) "as compared with healthy women" (22.8±4.78 vs. 16.9±3.19 pg/ml)"."The study demonstrated thatIL-23 was significantly decreased in follicular fluid of PCOS women" (P. value 0.008) "as compared with healthy women" (7.19±1.71 vs. 13.71±1.92 pg/ml). It was concluded that IL-10and IL17A were elevated in PCOS women while IL-12 and IL-23 were reduced as compared with control group.