Effect of Ethanolic Extract of Bauhinia variegata and Commiphora mukul in Regulating Thyroid Stimulating Hormone in Hypothyroidism Induced Albino Wistar Rats

Abstract

The present clinical interventions used for the management of the hypothyroidism are still associated with various side effects. In order to avoid this, the aim of the present investigation is to evaluate the effect of Bauhinia variegata and Commiphora mukul in regulating serum thyroid stimulating hormone levels in rats. Ethanolic extract of Bauhinia variegata (non-woody aerial parts) and Commiphora mukul(gum resin) was prepared via hot solvent extraction in a Soxhlet apparatus. Preliminary phytochemical screening showed that the ethanolic extract of Bauhinia variegates linn has all the phytoconstituents except phytosterol, fixed oil and fats. The Commiphora mukul gum resin extract showed the presence of all phytoconstituents except alkaloids, fixed oil and mucilage.The oral toxicity studies showed no abnormalities at the maximum tolerated dose level of 2000 mg/kg body weight. Methimazole were used to induce hypothyroidism in the rats. The animals were treated with different individual and combined dosages (100, 200 and 400 of two plant extracts. Serum TSH level analysis showed that a combination of high dose (400mg /kg body weight) of Bauhinia variegate and Commiphora mukul gum resin extracts showed a significant effect in lowering  serum TSH levels raised due to hypothyroidism. To conclude, a combined extract of plants could be a potential therapeutic alternative for the management of hypothyroidism. Keywords: Bauhinia variegate Linn., Commiphora mukul, Hypothyroidism, Thyroid stimulating hormones, Methimazole.