Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant

230Citations
Citations of this article
132Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Angiogcnesis is a fundamental vertebrate developmental process that requires signalling by the secreted protein vascular cndothclial growth factor-A (VEGF-A). VEGF-A functions in the development of embryonic structures, during tissue remodelling and for the growth of tumour-induced vasculature. The study of the role of VEGF-A during normal development has been significantly complicated by the dominant, haplo-insufficient nature of VEGF-A-targeted mutations in mice. We have used morpholino-based targeted gene knock-down technology to generate a zebrafish VEGF-A morphant loss of function model. Zebrafish VEGF-A morphant embryos develop with an enlarged pericardium and with major blood vessel deficiencies. Morphological assessment at 2 days of development indicates a nearly complete absence of both axial and interscgmcntal vasculature, with no or reduced numbers of circulating red blood cells. Molecular analysis using the endothelial markers fli-1 and flk-1 at 1 day of development demonstrates a fundamental distinction between VEGF-A requirements for axial and intersegmental vascular structure specification. VEGF-A is not required for the initial establishment of axial vasculature patterning, whereas all development of intersegmental vasculature is dependent on VEGF-A signalling. The zebrafish thus serves as a quality model for the study of conserved vertebrate angiogenesis processes during embryonic development. Copyright © 2000 John Wiley & Sons, Ltd.

Cite

CITATION STYLE

APA

Nasevicius, A., Larson, J., & Ekker, S. G. (2000). Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant. Yeast, 17(4), 294–301. https://doi.org/10.1002/1097-0061(200012)17:4<294::aid-yea54>3.0.co;2-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free