Suppression of platelet aggregation by Bordetella pertussis adenylate cyclase toxin

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Abstract

The effect of Bordetella pertussis adenylate cyclase toxin (ACT) on platelet aggregation was investigated. This cell-invasive adenylate cyclase completely suppressed ADP (10 μM)-induced aggregation of rabbit platelets at 3 μg/ml and strongly suppressed thrombin (0.2 U/ml)-induced aggregation at 10 μg/ml. The suppression was accompanied by marked increase in platelet intracellular cyclic AMP (cAMP) content and was diminished by the anti-ACT monoclonal antibody B7E11. A catalytically inactive point mutant of ACT did not show the suppressive effect. Since an increase of cAMP content is a known cause of platelet dysfunction, these results indicate that the observed platelet inactivation was due to the catalytic activity of ACT through increase of intracellular cAMP.

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Iwaki, M., Kamachi, K., Heveker, N., & Konda, T. (1999). Suppression of platelet aggregation by Bordetella pertussis adenylate cyclase toxin. Infection and Immunity, 67(6), 2763–2768. https://doi.org/10.1128/iai.67.6.2763-2768.1999

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