Determination of the Complete Nucleotide Sequence of Brevibacterium lactofermentum Plasmid pAM330 and Analysis of Its Genetic Information

Abstract

We have determined the complete nucleotide sequence of the plasmid pAM 330 DNA which is derived from Brevibacterium lactofermentum ATCC 13869, using the dideoxy chain termination method. It contains 4448 base pairs (bp), corresponding to a molecular weight of 3.0 mega-daltons. Computer-aided analysis of the sequence revealed eight open reading frames (ORFs). Some of these ORFs showed the presence of a canonical promoter and Shine-Dalgarno (SD) sequence upstream from the initiation codon and a terminator sequence downstream from the stop codon. © 1986, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.