Increased plasma levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in patients with pseudoexfoliation glaucoma

8Citations
Citations of this article
13Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Purpose. To investigate systemic oxidative stress-induced DNA damage in patients with pseudoexfoliation glaucoma (PXG), we estimated plasma levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) as a marker for oxidative DNA damage in comparison to controls. In addition, we also examined a combined effect of lysyl oxidase-like 1 (LOXL1) polymorphism status and 8-OHdG levels on PXG risk. Materials and Methods. A retrospective case-control study was performed to estimate plasma levels of 8-OHdG in 41 PXG patients and 45 nonglaucomatous controls using the enzyme-linked immunosorbent assay (ELISA). The assay was performed in duplicate on an automated ELISA analyzer. Two common polymorphisms (rs1048661 and rs3835942) in LOXL1 gene were genotyped by Sanger sequencing. Results. The mean and median levels of 8-OHdG were significantly increased in the PXG cases (p=0.032) and male subjects (p=0.041). Subjects with levels greater than the third quartile (75% percentile) exhibited a significant increased risk of PXG (odds ratio = 4.06, 95% confidence interval (CI = 1.11-14.80, p=0.029)). Within- and between-group comparisons showed that the mean levels were higher in individuals carrying the LOXL1 risk variant (G/G), but not statistically significant. In logistic regression analysis, both 8-OHdG (p=0.044) and rs3835942 (p=0.012) showed a statistically significant effect on the PXG outcome. However, the effect was lost when age, sex, and rs1048661 were included. A significant positive correlation was observed between 8-OHdG levels and intraocular pressure (R=0.284, p=0.008) and cup/disc ratio (R=0.233, p=0.031). Furthermore, in receiver operating characteristic analysis, the area under the curve was statistically significant (p=0.032) with a value of 0.635 (95% CI = 0.518-0.751). Conclusion. The study demonstrates an association of increased plasma levels of 8-OHdG in patients with PXG, supporting the role of oxidative stress, and increased oxidative DNA damage in PXG development.

References Powered by Scopus

8-Hydroxy-2′ -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis

1601Citations
N/AReaders
Get full text

Biomarkers of oxidative damage in human disease

1446Citations
N/AReaders
Get full text

The Role of the Reactive Oxygen Species and Oxidative Stress in the Pathomechanism of the Age-Related Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults

1160Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Oxidative stress in optic neuropathies

43Citations
N/AReaders
Get full text

Elevated Plasma Level of 8-Hydroxy-2′-deoxyguanosine Is Associated with Primary Open-Angle Glaucoma

14Citations
N/AReaders
Get full text

Current understanding of genetics and epigenetics in pseudoexfoliation syndrome and glaucoma

8Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Kondkar, A. A., Sultan, T., Azad, T. A., Tabussum, L., Osman, E. A., & Al-Obeidan, S. A. (2019). Increased plasma levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in patients with pseudoexfoliation glaucoma. Journal of Ophthalmology, 2019. https://doi.org/10.1155/2019/8319563

Readers over time

‘19‘20‘21‘22‘2402468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 3

38%

Professor / Associate Prof. 2

25%

Researcher 2

25%

Lecturer / Post doc 1

13%

Readers' Discipline

Tooltip

Medicine and Dentistry 3

38%

Nursing and Health Professions 2

25%

Biochemistry, Genetics and Molecular Bi... 2

25%

Chemistry 1

13%

Save time finding and organizing research with Mendeley

Sign up for free
0