Preclinical toxicological screening of Siddha medicine Ganthi Mezhugu by acute toxicity studies in Wistar albino rats

Abstract

Introduction and Aim: Siddha formulations of drugs had been used for hundreds of years in international conventional drugs for his or her capacity fitness benefits. Ganthi Mezhugu (GM) has a wide range of therapeutic properties. The objective of the present research was to establish the safety profile of the experimental drug GM in a rat model by acute and sub-acute oral toxicity, as per OECD regulatory guidelines. Materials and Methods: Acute toxicity testing is done for an animal species to determine the effects of a single dose on that species. In this investigation, acute toxicity was assessed using single oral administrations of 50 mg/kg, 300 mg/kg, and 2000 mg/kg. In the subacute investigation, the test substance GM was repeatedly dosed (4.68, 23.4, and 46.8 mg/kg/day) for 28 days followed by these hematological and biochemical parameters were assessed. Results: The study's findings showed that after administering the test substance GM to experimental animals once or repeatedly, there was no evidence of toxicity and no deaths. The subacute toxicity study's GM treated groups' normal hematological and serological profiles provided additional evidence that the formulation is not harmful. Conclusion: The finding of the study supported the notion that Siddha formulation GM is non-toxic and has a broad spectrum of safety.