Cytochalasin D inhibits smooth muscle contraction by directly inhibiting contractile apparatus

Abstract

We investigated the mode of relaxant effects of cytochalasin D, a capping agent of actin filaments, on contractile responses in the rat aorta and chicken gizzard smooth muscles. Cytochalasin D inhibited the contraction induced by high K+ or noradrenaline (10 nM-1 μM) without changing cytosolic Ca2+ level ([Ca2+](i)) in the rat aorta. In the absence of external Ca2+, 12-deoxyphorbol-13-isobutylate (DPB) (1 μM) induced sustained contraction without increasing in [Ca2+](i) and cytochalasin D also inhibited this contraction. In the permeabilized chicken gizzard smooth muscle, cytochalasin D inhibited the Ca2+ (1-10 μM)-induced contraction. Cytochalasin D also inhibited the Ca2+ independent contraction in the muscle which had been thiophosphorylated by ATPγS. Cytochalasin D decreased the velocity of superprecipitation in the chicken gizzard native actomyosin (myosin B) affecting neither the level of MLC phosphorylation nor Mg2+ ATPase activity. These results suggest that cytochalasin D inhibits smooth muscle contractions without any effect on the Ca2+-dependent MLC phosphorylation or subsequent activation of myosin ATPase activity. Based on these evidences, it is concluded that cytochalasin D may inhibit smooth muscle contraction possiblly through uncoupling of the force generation from the activated actomyosin Mg2+ ATPase.