Liquid Chromatography–tandem Mass Spectrometry Method for Determination of Paclitaxel in Human Plasma

Abstract

A rapid and sensitive liquid chromatography – tandem mass spectrometry (LC-MS/MS) method for determination of the Paclitaxel in human plasma was developed and validated.Placitaxel was extracted from plasma by a two-step extraction procedure with using of chloroform as Liquid –Liquid extractive organic solvent. LC-MS/MS analysis using electro-spray ionization (ESI+) was performed on Phenomenex C-18 Column (250x1.5 μ ) using as acetonitrile, water (80:20+0.1% acetic acid) as mobile phase. The method has a fl ow rate of 0.8ml/min. Retention of Paclitaxel was 4.60 minutes.An excellent linearity (r 2 .099) between the peak ratios and Paclitaxel concentrations over the range of 10-100 ng/ml of plasma was studied. The lower limit of detection for Paclitaxel on mass was 10 pg/mL. There was about 100 percent of Paclitaxel was recovered in extracted samples. The study of Paclitaxel standard and extraction standard calibration curves were useful in pharmacokinetics analysis.