Production of Chitinase by Solid State Fermentation from Rice Bran

Abstract

Statistics based experimental design on chitinase; production by Serratia marcescens was optimized in solid state; fermentation using Plackett-Burman design and Response; surface methodology. The important medium components; identified by initial screening method of Plackett-Burman were; colloidal chitin; yeast extract; MgSO4.7H2O and KH2PO4.; Plackett-Burman Pareto chart illustrates the order of; significance of the variables affecting the cellmass production.; Central composite response surface methodology was; performed to evaluate the effects of temperature; pH; inoculum size and substrate concentration on production of; chitinase by Serratia marcescens was studied using rice bran; under solid state fermentation. Statistical analysis of results; showed that; the linear and quadric terms of these four; variables had significant effects and evident interactions; existing between pH and substrate concentration were found; to contribute to the response at a significant level. Under the; conditions of pH (5); Temperature (35o; C); inoculum size (1.8%); and substrate concentration (2.5 g/l) in the experiment. The; predicted response for chitinase production was 61.12 U/ml