Fluorescence-based functional assay for Wnt/β-catenin signaling activity

Abstract

Aberrant activation of β-catenin signaling has been implicated in the development of human cancer. As a Wnt signal transducer, β-catenin forms a complex with the lymphocyte enhancer-binding factor/T cell factor transcription factor and activates downstream targets that promote cell proliferation. Here we developed a Wnt-dependent β-catenin-mediated heterologous transactivation system, which consisted of a chimeric transcription factor constructed by fusing the GAL4 DNA-binding domain with the full-length β-catenin, and a GAL4-responsive reporter expressing GFP. The chimeric transcription factor was highly unstable and exerted no detectable transactivating effect on the GAL4-responsive reporter. However, lithium and Wntl significantly stabilized this chimeric transactivator, indicating that this transactivation system is regulated by β-catenin in a Wnt-responsive fashion. Thus, this transactivation system could be used as a functional reporter to identify potential upstream factors that deregulate β-catenin signaling during tumorigenesis, as well as to screen for potential anti-cancer agents that specifically inhibit β-catenin signaling in human tumors.