Traveling NADH and proton waves during oscillatory glycolysis in vitro

Abstract

Propagation and mutual annihilation of circular and spiral NADH and proton waves were detected by spatially resolved spectrophotometry and fluorescent proton indicators in a biological in vitro system: an organelle-free yeast extract. Spontaneous wave generation during glycolytic sugar degradation is established after an induction period of about 1 h. Controlled wave initiation could be performed by local injection of the strong activator of phosphofructokinase, fructose 2,6-bisphosphate. A crucial role for wave initiation and control of pattern dynamics is attributed to the key enzyme of glycolysis, the allosterically regulated phosphofructokinase. An overall increase in the concentration of its positive effector AMP leads to the formation of rotating spirals. The dynamics of the observed wave patterns resemble that of self-organized calcium waves as recently found in frog eggs and heart cells.