Assignment of PCR markers to river buffalo chromosomes

Abstract

In the process of developing a buffalo physical map using somatic cell hybrids and the cattle gene map as a template, ten PCR primers designed for four coding genes: F10, FSHB, HBB, and CYM and six DNA segments: TGLA9, TGLA227, UWCA5, CSSM6, CSSM47 and RF131 were tested on a panel of 47 buffalo- hamster somatic cell hybrids. F10-TGLA9, FSHB-HBB and UWCA5-TGLA227, respectively, were found to segregate together forming three syntenic groups. These three syntenic groups have also been reported in cattle, where they have been assigned to chromosomes BTA 12, BTA 15 and BTA 18, respectively. Comparative mapping predicts the assignment of these syntenic groups to river buffalo chromosomes BBU 13, BBU 16 and BBU 18, respectively.