Measurement of Helicobacter pylori Using Anti Its Urease Monoclonal Antibody by Surface Plasmon Resonance

Abstract

Adults over 70% of Japanese are infected by Helicobacter pylori causing chronic gastrisis and gastric ulcer. In this study, by employing a unique monoclonal antibody against Helicobacter pylori urease, the bacterium was detected down to 2 × 107 cell/ml using a SPR apparatus equipping two detecting cells. Ultrasonicated sample of Helicobacter pylori gave a huge improvement in the detection limit rather than the non-ultrasonicated sample by 100 fold. Escherichia coli was hardly detected, suggesting the specific detection of Helicobacter pylori by this measurement system. Using this type of SPR apparatus, the wider concentration of Helicobacter pylori could be detected comparing with the conventional assay kit, while the detection limit of the SPR system was worse by 10 fold.