Effect of inoculum morphology on production of Nigerloxin by solid state fermentation

Abstract

Nigerloxin (2-amido-3-hydroxy-6-methoxy-5-methyle-4-(prop-1'enyl) benzoic acid) is a Lipoxygenase and Aldose reductase inhibitor produced by Aspergillus niger MTCC 5116. It is produced only under solid state fermn. (SSF) and the inoculum required for the inhibitor prodn. is developed in submerged condition. As this is a newly discovered enzyme inhibitor with potential com. success against diabetic complications, such as neuropathy and cataract, its complete fermn. process parametric study is not reported. In this study, the role of phys. parameters (spore suspension, initial pH, incubation temp. and agitation) in spore germination and pellet size in inoculum development broth in submerged fermn. for the enhanced prodn. of nigerloxin through SSF has been studied. It was concluded that 500 μl of spore suspension in the inoculum development broth at pH 7, incubated at 30°C and 200 rpm gave an ideal pellet size 1.23 mm resulting in 6.0 mg of nigerloxin/g dry wt. of wheat bran in SSF. [on SciFinder(R)]