Molecular detection of some virulence genes of Enterococcus faecalis isolated from different clinical samples in Babylon province/ Iraq

Abstract

A total of 120 clinical samples were collected during this study which obtained from patients suffering from different infection such as UTI, vaginitis, wound infection, gastro enteritis who admitted to two main hospitals of AL-Hilla City: Al-Hilla Surgical Teaching Hospital and Al-Hashimiyah General Hospital during a period extending from (Ougust 2021 to November 2021). All sample were subjected to aerobic culturing on different media and it was found that out of the total 120samples, 87 (72.5%) samples showed positive bacterial culture. No growth was seen in other 33(27.5%) samples which indicate the presence of microorganisms that may be cultured with difficulty such as virus, fungi and other agent or may be due to difference in the size and nature of the samples. Among (87) positive culture were culturing on chromogenic ager medium (selective media), 20(22.98%)positive samples was identified as E. faecalis. Identification of E. faecalis depends mainly on the cultural, and biochemical characteristics and also microscopic patterns. These isolates then subjected to molecular detection method using specific primer based on D-alanine D-alanine ligase gene as a genetic marker for confirmed identification of E. faecalis by PCR, the results revealed that 20(100%) were positive for ddl .