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Shuttle mutagenesis of Legionella pneumophila: Identification of a gene associated with host cell cytopathicity

Infection and Immunity
DOI: 10.1128/iai.62.9.4075-4080.1994
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Abstract

We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.

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Arroyo, J., Hurley, M. C., Wolf, M., McClain, M. S., Eisenstein, B. I., & Engleberg, N. C. (1994). Shuttle mutagenesis of Legionella pneumophila: Identification of a gene associated with host cell cytopathicity. Infection and Immunity. https://doi.org/10.1128/iai.62.9.4075-4080.1994

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