Cytotoxicity Test of Chitosan Solution as Acrylic Denture Cleansers Solution

Abstract

Removable denture is the easiest and most affordable management for tooth loss. Denture cleanser solution is needed in daily care procedure of removable denture but the price is relatively expensive. Chitosan is a solution derived from chitin marine animals. The objective of the study is to test cytotoxic effects of Chitosan in various concentrations. We used 13 samples treated with chitosan and acetic acid in different concentration. Cytotoxicity test was done with MTS assay method in-vitro on BJ cells (normal skin cells). The results showed that percentage of cell death (cytotoxicity effect) in chitosan solution increased as the concentration raised, with IC50 values of 0.032% chitosan solution. We concluded that chitosan is cytotoxic and unsuitable to be used as removable denture cleanser solution.