Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells

Abstract

This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP-1-1) in RAW264.7 cells. SCP-1-1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP-1-1 could significantly enhance phagocytic ability, NO production, and some cytokines (TNF-α, IL-6, and IL-1β) secretion of RAW264.7 cell in a dose-dependent manner. Western blot analysis results demonstrated that SCP-1-1 could regulate the expression levels of the key proteins in the signaling pathways of RAW264.7 cell and might associated with NF-κβ and PI3K signaling pathway. These findings could contribute to elucidate the immunomodulatory activities of the polysaccharide from Sinonovacula constricta.