Improving the Efficiency and Antioxidant Activity of Essential Oil Extraction from Abies sachalinensis by Underwater Shockwave Pretreatment for the Construction of Low-Energy and Sustainable Essential Oil Extraction System

Abstract

Essential oils (EOs) from Abies sachalinensis (Sakhalin fir), a conifer species found in Sakhalin Island and Hokkaido in Japan, effectively remove nitrogen dioxide and possess antifungal activity. EOs also exert a relaxing effect and enhance air quality. Underwater shock waves generate instantaneous high pressure that ruptures cell walls, enhancing the performance of steam distillation and oil extraction. In this study, we aimed to increase the yield and quality of A. sachalinensis extracts using shockwaves. Leaves and branches were subjected to shockwave pretreatment or left untreated before EO extraction by steam distillation. EO yield of untreated dried leaves was 2.4 g/kg of dry leaf weight (DW). Upon application of a 3.0 kV, 3.6 kJ shockwave, the yield increased with the number of shockwave cycles. After ten cycles, yield increased 13.6-fold. Pretreatment with shockwaves for 10 cycles resulted in approximately 6- and 13-fold reductions in total energy consumption relative to fresh and dried leaves, respectively. Antioxidant activity increased more than 30-fold in shockwave-pretreated leaves than in untreated dried leaves after 10 cycles. This novel process can significantly reduce the energy used for EO extraction in steam distillation, thereby contributing to the development of a sustainable, low-energy EO production system.