Optimization of Biomass of Keratinase Producing Bacillus sp CBNRBT2 to Utilize in Whole Cell Immobilization for Feather Degradation

Abstract

Bacillus sp CBNRBT2, obtained from Centre for Bioscience and Nanoscience Research, was used in this study. Initially, biomass was optimized for the incubation period, pH, and temperature, following biomass optimization with RSM. The obtained organism was able to produce keratinase, which was found to be a 64 KDa protein. Now the biomass produced using the optimized condition was immobilized and checked for its ability to degrade keratinase. Efficiency was compared with the whole organism. Immobilized organisms were degrading keratin and feather better. It was found to reduce featherweight by 41 %. Thus, this immobilized bacterium can be used for poultry waste treatment.