Zero echo time17o-mri reveals decreased cerebral metabolic rate of oxygen consumption in a murine model of amyloidosis

Abstract

The cerebral metabolic rate of oxygen consumption (CMRO2 ) is a key metric to investigate the mechanisms involved in neurodegeneration in animal models and evaluate potential new thera-pies. CMRO2 can be measured by direct17O magnetic resonance imaging (17O-MRI) of H217O signal changes during inhalation of17O-labeled oxygen gas. In this study, we built a simple gas distribution system and used 3D zero echo time (ZTE-)MRI at 11.7 T to measure CMRO2 in the APPswe/PS1dE9 mouse model of amyloidosis. We found that CMRO2 was significantly lower in the APPswe/PS1dE9 brain than in wild-type at 12–14 months. We also estimated cerebral blood flow (CBF) from the post-inhalation washout curve and found no difference between groups. These results suggest that the lower CMRO2 observed in APPswe/PS1dE9 is likely due to metabolism impairment rather than to reduced blood flow. Analysis of the17O-MRI data using different quantification models (linear and 3-phase model) showed that the choice of the model does not affect group comparison results. However, the simplified linear model significantly underestimated the absolute CMRO2 values compared to a 3-phase model. This may become of importance when combining several metabolic fluxes measurements to study neuro-metabolic coupling.