Soluble and Bound forms of Aminopeptidase in hen's Egg Yolk

Abstract

An aminopeptidase, Ey, is a newly discovered enzyme of hen's egg yolk. About 95% of the total activity was found in the yolk plasma fraction, with most of the remainder in the yolk granule fraction. The highly purified fraction from plasma showed about 13,000-fold higher specific activities than that of yolk plasma. Polyacrylamide gel electrophoresis revealed that the enzyme was homogeneous. The bound enzyme in the granule was extracted with 3% NaCl and was partially purified. The soluble and bound forms of aminopeptidase differ in solubility, molecular weight, heat stability, optimal pH, and Km. © 1989, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.