Determination of bacteriophage ETEC-phage-TG to control pathogenic Escherichia coli

Abstract

Objectives: This study aimed to isolate lytic bacteriophage to control EHEC and ETEC, characterize, and apply their ability to control these two bacteria in food. Results: One of the bacteria that contaminate the food is Diarrheagenic Escherichia coli (DEC) which was consist of Enterohaemorrhagic E. coli and Enteropathogenic E. coli. Preservation methods were used to improve shelf-life to reduce microbial growth but it might have side effect to the nutrition and human health. Therefore, it is required to explore an alternative method that can be used to be a biocontrol agent for food, including the bacteriophage approach. Our study showed that these two phages are promising to be used as biocontrol agent in food. Genomic DNA sequencing performed that this phage being exclusively lytic.