Histochemical characteristics of the vitellogenic oocytes of the bluefin tuna, Thunnus thynnus L.

Abstract

Very little information is available with respect to dynamic aspects of oocyte development and to yolk composition of tuna species. Since histological approaches can offer considerable information about the ovarian development of fish, and because different cytochemical/immunohistochemical tests are suitable tools for proving the presence of different macromolecules in several organs/tissues/cells, they can be good biomarkers of the reproductive mechanism in fish. The present study investigates the presence and distribution of carbohydrates, proteins, glycoconjugates, lipids, calcium, iron and vitellogenin in the bluefin tuna, Thunnus thynnus oocytes during the synthesis of yolk (globules and granules) in the vitellogenic phase. During the initial vitellogenic phase, oocytes (diameter: 249 ± 21.87 mm) contain oil globules (or vacuoles) which consist exclusively of neutral lipids. During this phase very small yolk granules appear around the cytoplasm. Glycolipids, phospholipids, glycoproteins (Nacetyl- D-galactosamine –GalNAc– sugar residues), calcium, proteins rich in tyrosine, tryptophan, lysine, arginine, cysteine and cystine, as well as vitellogenin are the main constituents of the yolk granules and the follicular envelope of these real vitellogenic oocytes (diameter: 386 ± 79.04 mm). The intergranular cytoplasm of the vitellogenic oocytes also contain glycogen and glycoconjugates with Mannose –Man– and/or Glucose –Glc–; N-acetyl-D-galactosamine –GalNAc–; L-Fucose –Fuc– and Nacetyl- D-glucosamine –GlcNAc– and/or sialic acid –NANA– sugar residues. The zona radiata is mainly composed of proteins; in this cellular layer, scarce neutral glycoproteins and lipids (phospholipids/glycolipids) are also present.