Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite

Abstract

Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery of a pure product at low costs. This study presents a simplified method for large scale poly(3-hydroxybutyrate), poly(3HB), extraction using sodium hypochlorite. Poly(3HB) was extracted from cells of Ralstonia eutropha H16 at almost 96% purity. At different extraction volumes, a maximum recovery rate of 91.32 % was obtained. At the largest extraction volume of 50 L, poly(3HB) with an average purity of 93.32 % ± 4.62 % was extracted with a maximum recovery of 87.03 % of the initial poly(3HB) content. This process is easy to handle and requires less efforts than previously described processes. © 2012 Heinrich et al.