The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E2 to the culture medium. The organoids are accompanied by myofibroblasts which become visible in the next 2 days of culture. The intestinal enteroids (free of myofibroblasts) can be obtained from adult chicken intestine.
CITATION STYLE
Pierzchalska, M., Panek, M., Czyrnek, M., & Grabacka, M. (2019). The three-dimensional culture of epithelial organoids derived from embryonic chicken intestine. In Methods in Molecular Biology (Vol. 1576, pp. 135–144). Humana Press Inc. https://doi.org/10.1007/7651_2016_15
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