Biodegradation of premium motor spirit (PMS) by lipase from Bacillus thuringiensis and Lysinibacillus sphaericus

Abstract

This study reported production of lipase by Bacillus thuringiensis and Lysinibacillus sphaericus. Bacteria isolates were screened on Bushnell-Hass Mineral Salt medium containing 1% PMS for oil degradation. Two potent isolates were identified using 16S rRNA as Bacillus thuringiensis and Lysinibacillus sphaericus. They were cultured for lipase production in a submerged medium. The crude lipases extracted were used for degradation of PMS. Optimum degradation of PMS 44.5% and 37.4% were obtained by lipase from Bacillus thuringiensis and Lysinibacillus sphaericus respectively at pH 7 and 35 in 20 days. This study therefore presented the use of bacterial lipase in degradation of PMS as a simple and effective approach.Keywords: Bacillus spp., biodegradation, lipase,