Antibiotic resistance and methicillin resistant Staphylococcus aureus isolated from bovine, crossbred etawa goat and human

Abstract

Antimicrobial resistance patterns and gene encoding for methicillin/oxacillin resistance (mecA) were determined in 73 Staphylococcus aureus. The isolates of S aureus originated from bovine (39 isolates) from Peranakan Etawa (PE) or crossbred Etawa goats (24 isolates) and from patients of Sarjito hospital (10 isolates) in Indonesia. The identification ofS aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene and thermonuclease (nuc) genes. Staphylococcus aureus originating from human and bovine were more resistant than those of goat origin. Seventeen S aureus(23.29%) were resistant to single antibiotic and 15 isolates (20.55%) showed resistance to two antimicrobial agents. Multi resistances were found in 26 (35.62%) ofS aureus i solates. Resistance to ampicillin was the most common finding (80, 76.92 and 41.67%), followed by gentamicin (30,51.28 and 25%), oxacillin (50, 38.46 and 58.33%), tetracyclin (40, 28.21 and 16.67%) and erythromycin (40, 23.08 and 20.83%) for human, bovine and goat, respectively. By PCR amplification could be observed in 5 (12.82%) methicillin/oxacillin resistant (mecA) genes for bovine isolates, 1 (4.17%) goat isolates and 9 (90%) human isolates. These isolates were identified as methicillin resistant S aureus(MRSA). Most of MRSA were resistant to oxacillin (60%), ampicillin (66.7%), tetracyclin (40%), erythromycin (33.3%) and gentamicin (20%) The resistancy of S aureusto methicillin/oxacillin and the other antibiotics in the present study might help to understand the distribution of methicillin resistant S aureus (MRSA) among human bovine and goat isolates and might help to control S aureus infections.