Detection of polymorphic patterns of genomic DNA amplified by RAPD-PCR in sweet potato weevils, Cylas formicarius (Fabricius) (Coleoptera: Brentidae)

Abstract

The polymorphic patterns of genomic DNA amplified by RAPD-PCR were detected in laboratory-cultured populations of sweet potato weevils, Cylas formicarius collected from the Southwest islands (Nansei-Shoto) of Japan. When three sets of primers, T13/T13, T05/T05 and T01/T07 were used for PCR, the polymorphic patterns of the amplified DNA were classified into nine types. Of these types, A1B1C3 was common in all populations tested. The present study determined the useful primer sets that will enable the successful classification of sweet potato weevils based upon t the polymorphic patterns of genomic DNA amplified by RAPD-PCR.