Simultaneous quantification of mrna and protein in single cells reveals post-transcriptional effects of genetic variation

31Citations
Citations of this article
103Readers
Mendeley users who have this article in their library.

Abstract

Trans-acting DNA variants may specifically affect mRNA or protein levels of genes located throughout the genome. However, prior work compared trans-acting loci mapped in separate studies, many of which had limited statistical power. Here, we developed a CRISPR-based system for simultaneous quantification of mRNA and protein of a given gene via dual fluorescent reporters in single, live cells of the yeast Saccharomyces cerevisiae. In large populations of recombinant cells from a cross between two genetically divergent strains, we mapped 86 trans-acting loci affecting the expression of ten genes. Less than 20% of these loci had concordant effects on mRNA and protein of the same gene. Most loci influenced protein but not mRNA of a given gene. One locus harbored a premature stop variant in the YAK1 kinase gene that had specific effects on protein or mRNA of dozens of genes. These results demonstrate complex, post-transcriptional genetic effects on gene expression.

Cite

CITATION STYLE

APA

Brion, C., Lutz, S. M., & Albert, F. W. (2020). Simultaneous quantification of mrna and protein in single cells reveals post-transcriptional effects of genetic variation. ELife, 9, 1–34. https://doi.org/10.7554/eLife.60645

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free