DNA isolated from Fusarium lateritium Nees: Fr.-infected 'Jewel' sweetpotato [Ipomoea batatas (L.) Lam.] plants was compared to F. lateritium-free 'Jewel' plants for differences in random amplified polymorphic DNA (RAPD) marker products. Differences in RAPD marker products were detected. Amplified DNA isolations from F. lateritium-infected 'Jewel' plants generated additional, unique DNA fragments not found in amplified DNA isolations of F. lateritium-free 'Jewel' plants. These unique amplified DNA fragments were consistent with those obtained from amplified DNA isolations of the F. lateritium isolate, 91-27-2, used for inoculation. We found that F. lateritium DNA successfully competes with sweetpotato DNA in the polymerase chain reaction for priming sites in a 3 : 1 ratio of sweetpotato DNA to F. lateritium DNA. Our results indicate the importance of avoiding plant material infested with pathogens to avoid spurious marker bands.
CITATION STYLE
Buffone, F. A., La Bonte, D. R., & Clark, C. A. (1996). Random amplified polymorphic DNA markers in sweetpotato infected with Fusarium lateritium. HortScience, 31(7), 1215–1216. https://doi.org/10.21273/hortsci.31.7.1215
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