A Multifunctional Acoustic Tweezer for Heterogenous Assembloids Patterning

Abstract

Acoustic tweezers, capable of complicated manipulation of bioparticles by acoustic radiation forces using a noninvasive and noncontact approach, are an innovative technology for patterning assemble organoids. Hence, acoustic tweezers exhibit considerable potential for forming programmable patterning of organoids with specific spatial structures. Furthermore, heterogeneous assembloids with complex arrangement patterns can be built through sequential assembling and culturing to explore polarized tissue development or disease metastasis in multiple organs. This study focuses on the structural assembly of organoids using an ultrasonic 2D matrix array to generate real-time switching of different acoustic fields. In addition, a local renal injured assembloid is fabricated to study and verify its application in tissue engineering and disease modeling.