Triple-Helical Target Sites of Oncogenes to Direct Therapeutic Triplex-Forming Oligonucleotides for the Management of Colorectal Cancer

Abstract

The triple helix formation is an intranuclear process. The therapeutically targeted Triplex-Forming oligonucleotides (TFOs) can bind to Triplex Targeted Sites (TTS) located on double-stranded DNA and subsequently can inactivate a gene or can induce a mutation. The current study is intended to identify the total TTS in the human genome along with the number of TTS at promotor and protein-coding regions of genes, oncogenes and narrow down the TTS associated with oncogenes that are responsible for colorectal cancer (CRC). The TTS Mapping and integration (TTSMI) database is used to identify the TTS sites. The total number of TTS in the human genome were retrieved, segregated the TTS located on oncogenes, total number of oncogenes associated with TTS sites and sequences with respect to CRC was tabulated. The identified TTS sequences can be utilized to develop TFOs to target corresponding oncogenes associated with CRC.