Suplementasi Ekstrak Daun Ketapang (Terminalia catappa) pada Potato Sucrose Agar (PSA) untuk Menggantikan Dichloran Rose Bengal Chloramphenicol (DRBC) pada Praktikum Mikrobiologi Pangan dan Pengolahan

Abstract

Ketapang (Terminalia catappa) trees are commonly utilized as shade plants. In addition to their beneficial seeds, their leaf extracts also possess antimicrobial properties. Aquous leaf extract of ketapang, exhibits antifungal activity. The aim of this study is to apply ketapang leaf extract on Potato Sucrose Agar (PSA) medium for Microbiology of Food and Processing laboratory experiments on food spoilage fungi testing. Rhizopus sp. fungi were inoculated on PSA medium containing young and old ketapang leaf extracts (at concentrations of 10% and 20%). The fungi were then incubated at room temperature for 4 days, and their growth diameter was measured daily to determine the inhibitory effect of the extract. Dichloran Rose Bengal Chloramphenicol (DRBC) medium was used as a control medium, as utilized in food spoilage fungi testing experiments. The research results indicate that the highest inhibitory effect was obtained with 20% young ketapang leaf extract. The inhibitory effect decreases with lower concentrations of ketapang leaf extract; it decreased by 17% after heat sterilization. Application of food spoilage fungi testing using corn samples revealed similar contamination levels of seeds and fungal genera growth on PSA medium compared to DRBC medium. Therefore, the addition of 20% ketapang leaf extract to PSA medium can be utilized as a substitute for DRBC medium in food spoilage fungi testing