Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics

Abstract

(Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.