VARIAN GENETIK Sardinella lemuru DI PERAIRAN SELAT BALI

Abstract

Sardinella lemuru is one of the fish species that dominate in the Bali Strait. Identification Sardinella lemuru with the others Sardinella in the Strait of Bali is based on morphological characteristics, whereas genetic identification of Sardinella lemuru in Bali Strait has not been done and variations genetic also unknown. This research aims to ascertain the type of Sardinella lemuru in the bali strait and Determine genetic variation and kinship based on cytochrome oxidase subunit (COI) gene. The method is performed by isolating DNA from fish organs and the results are amplified by the COI gene sequencing then performed to obtain the data sequence of Sardinella lemuru Bali Strait and analysed using the program MEGA 5.2. Results showed that lemuru in Bali Strait is Sardinella lemuru species with the degree of similarity BLAST analysis of 98-100%. 11 samples sequence of lemuru forming two large clad. The results also showed that the species of Sardinella lemuru and Sardinella longiceps different species with genetic distance> 0,019.