Bacteriocin from Donggala cow's milk againts Salmonella typhi

Abstract

Bacteriocin is a protein compound produced by lactic acid bacteria (LAB) and able to inhibit the growth of pathogenic bacteria. Bacteriocin was isolated from donggala cow's milk (endemic cow of central sulawesi) which is not commercialized. This study was the first report conducted in central sulawesi. The aim of this study was to determine the ability of bacteriocin inhibiting Salmonella typhi. The experiments were selection of antimicrobial LAB, activity and stability (temperature and pH) of crude extract of bacteriocin using well diffusion method. There were 9 isolates of LAB and characterized by a clear zone around the colony on MRSA + CaCO3 1% media. The result showed that was one isolate (MA 9) having inhibitory activity against S. typhi at pH 5 (acid) was 1,3mm and pH 7 (neutral) was 3,17mm. Optimum bacteriocin inhibits S. typhi at 37°C and pH 7 (inhibitory activity against S. typhi was 2,58mm). Bacteriocin has a potential as an alternative natural preservative.