Influence of culture media on detection of carbapenem hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Abstract

In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrixassisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.