Chlorination of schistosoma mansoni cercariae

Abstract

Background Schistosomiasis is a water-based disease acquired through contact with cercaria-infested water. Communities living in endemic regions often rely on parasite-contaminated freshwater bodies for their daily water contact activities, resulting in recurring schistosomiasis infec-tion. In such instances, water treatment can provide safe water on a household or community scale. However, to-date there are no water treatment guidelines that provide information on how to treat water containing schistosome cercariae. Here, we rigorously test the effectiveness of chlorine against Schistosoma mansoni cercariae. Method S. mansoni cercariae were chlorinated using sodium hypochlorite under lab and field condi-tion. The water pH was controlled at 6.5, 7.0 or 7.5, the water temperature at 20˚C or 27˚C, and the chlorine dose at 1, 2 or 3 mg/l. Experiments were conducted up to contact times of 45 minutes. 100 cercariae were used per experiment, thereby achieving up to 2-log10 inacti-vations of cercariae. Experiments were replicated under field conditions at Lake Victoria, Tanzania. Conclusion A CT (residual chlorine concentration x chlorine contact time) value of 26±4 mg.min/l is required to achieve a 2-log10 inactivation of S. mansoni cercariae under the most conserva-tive condition tested (pH 7.5, 20˚C). Field and lab-cultivated cercariae show similar chlorine sensitivities. A CT value of 30 mg.min/l is therefore recommended to disinfect cercaria-infested water, though safety factors may be required, depending on water quality and oper-ating conditions. This CT value can be achieved with a chlorine residual of 1 mg/l after a contact time of 30 minutes, for example. This recommendation can be used to provide safe water for household and recreational water activities in communities that lack safe alterna-tive water sources.