Oryzalin-induced chromosome doubling in Buddleja to facilitate interspecific hybridization

Abstract

A protocol for producing fertile tetraploid forms of the hybrid Buddleja madagascarensis Lam. x B. crispa Benth. would enable introgression of orange flower, pubescence, and nondehiscent fruit characteristics found in section Nicodemia (Tenore) Leeuw. into B. davidii Franchet section Buddleja. Excised nodal sections of a single sterile diploid selection from that cross were treated in vitro with 3, 5, or 7 μM oryzalin concentrations for 1, 2, or 3 days or were left as an untreated control. A population of plants was generated from these cultures and transferred to the greenhouse. Treated plants were initially screened phenotypically for higher ploidy levels on the basis of stem thickness and leaf size. Those selected based on polyploidy characteristics were subjected to cytometric analysis, confirming that six tetraploid plants were generated. Nodal survival rates were dependent on oryzalin concentration and treatment duration. Significant increases in fertility accompanied polyploidy induction, because crosses between the newly developed tetraploids and B. davidii cultivars produced viable fertile plants. Chemical name used: 3,5-dinitro-N4,N4-dipropylsulfanilamide (oryzalin).