Multiple paternity in fruits of Ipomopsis aggregata (Polemoniaceae)

Abstract

Two different mechanisms can result in multiple paternity within fruits: deposition of a mixed pollen load due to carryover of pollen from flower to flower and multiple pollinator visits in close succession. I investigated the extent of multiple paternity within fruits of Ipompsis aggregata containing from 2 to 14 seeds. A paternity analysis based on ten polymorphic isozyme markers revealed multiple paternity in a minimum of 68% (based on simple paternity exclusion) and up to 100% (based on identification of the most likely father) of the multiseeded fruits. The estimated number of fathers increased with the number of seeds in a fruit, with an average of four sires, and up to nine sires, represented in a single fruit. To explore whether this level of multiple paternity could be explained solely by simultaneous deposition of a mixed pollen load I constructed a computer simulation model based on previous measurements of movement patterns and pollen carryover by the hummingbird pollinators. Model predictions provided a good match to observed values for number of sires per fruit. Thus the extensive pollen carryover in this species and consequent mixed pollen loads can explain the high levels of multiple paternity in natural populations.