Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins

Abstract

Background: Many enzymes of industrial interest are not in the market since they are bioproduced as bacterial inclusion bodies, believed to be biologically inert aggregates of insoluble protein. Results: By using two structurally and functionally different model enzymes and two fluorescent proteins we show that physiological aggregation in bacteria might only result in a moderate loss of biological activity and that inclusion bodies can be used in reaction mixtures for efficient catalysis. Conclusion: This observation offers promising possibilities for the exploration of inclusion bodies as catalysts for industrial purposes, without any previous protein-refolding step. © 2005 Garcïa-Fruitós et al; licensee BioMed Central Ltd.