Paper-Based Biosensor System for Fast and Sensitive Phenolic Compounds Detection

Abstract

In this original paper, the development of a paper-based, sensitive, low-cost, quantitative and consistent biosensor system was demonstrated for the suitable “naked-eye” detection of phenolic compounds (specifical catechol). The Paper-based system depends on the enzymatic color-changing reaction that observed by using chromogenic agent of 4-AAP (4-aminoantipyrine) which has a specific color changing reaction in the presence of horseradish peroxidase (HRP) enzyme, phenol and H2O2. The visual result was associated with the catechol concentration by using image processing software to evaluate the quantitative detection. The developed biosensor system demonstrated a linear detection range from catechol between 2,5 µM to 100 µM with 2.6% to 9.3 % of sd results. The detection limit was also calculated as 2,25 µM. Specificity, selectivity, stability and direct real sample analysis demonstrated the potential applicability of the developed paper-based biosensor system for catechol detection in food samples with minimal investment and an easy-to-use method.